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Updated: Aug 10, 2026
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ASCP ASCP-MLT Exam Overview:
| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Passing Score: | 400 (scale 0-999) |
| Exam Duration: | 150 minutes |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Real Exam Qty: | 100 |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Available Languages: | English |
| Related Certifications: | MLT(ASCPi) MLS(ASCP) PBT(ASCP) |
| Recommended Training: | ASCP BOC MLT Content Guideline ASCP Recommended Reading List |
| Exam Registration: | Pearson VUE Testing Center Scheduling ASCP BOC Official Application Portal |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance |
| Topic 2: Microbiology | 15-20% | - Specimen collection and culture techniques - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms |
| Topic 3: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Toxicology and liver/kidney function tests |
| Topic 4: Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Topic 5: Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures - Red blood cell physiology and disorders |
| Topic 6: Immunology and Serology | 5-10% | - Infectious disease serology - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies - Immune system principles and components |
| Topic 7: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Transfusion reactions and adverse events - Blood group systems and antigens - Blood components and storage - Antibody detection and identification |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Match each of the descriptions with the appropriate magnification:
1. Color, Rouleau, Overall Slide Quality, Cell Distribution
2. Platelet estimates RBC-platelet-WBC morphology WBC differential RBC inclusions
3. Select area to examine, WBC estimate
A) 10X
B) 100X (Oil)
C) 40X (Dry)
2. Hypochromia can best be described as:
A) Increase in nucleated RBCs
B) Abnormal erythropoiesis
C) Immature red blood cells
D) Decrease in Hgb concentration in RBCs
3. Which of the following statements applies to the venipuncture procedure?
A) Standard Precautions must be observed
B) the procedure includes preparation of the patient
C) selecting and preparing the puncture site is part of the procedure
D) all of the above
4. The anticoagulant present in a light-blue stopper tube is:
A) sodium citrate
B) potassium oxalate
C) Ethylenediaminetetraacetic acid (EDTA)
D) heparin
5. The proper pH for a unit of platelets must be above 6.2. According to the FDA, accurate pH measurement is time dependent, and samples should be tested within 1 hour of sampling, or as suggested by the manufacturer of the pH measurement system. It is recommended that a pH meter or gas analyzer be routinely used rather than pH (nitrazine) paper. However, if the laboratory chooses to determine pH measurements with nitrazine paper, the selected paper should read in increments of one-tenth units, or it may provide inaccurate measurements. Maintaining proper pH requires at least 35 ml, but preferably 50-70 ml of plasma per platelet unit.
In order to prevent a loss of viability in platelet concentrates during storage the pH must be maintained above:
A) 5.0
B) 7.5
C) 4.5
D) 6.2
Solutions:
| Question # 1 Answer: A,B,C | Question # 2 Answer: C | Question # 3 Answer: D | Question # 4 Answer: A | Question # 5 Answer: D |
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