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American Society of Microbiology ABMM Sample Questions:

1. During the processing of stool specimens for ova and parasite examination, the formalin-ethyl acetate concentration procedure primarily serves to:

A) Preserve parasite morphology and kill bacteria
B) Selectively culture protozoan trophozoites
C) Stain parasite nuclei for easier identification
D) Separate parasites from fecal debris and concentrate them


2. A patient develops a localized skin infection after being bitten by a tick. The infection is characterized by a slowly expanding erythematous rash with central clearing (erythema migrans). The MOST likely causative agent is:

A) Ehrlichia chaffeensis
B) Borrelia burgdorferi
C) Rickettsia rickettsii
D) Anaplasma phagocytophilum


3. An outbreak of gastroenteritis occurs among attendees of a company picnic. Epidemiological investigation points towards contaminated potato salad. Stool cultures from affected individuals grow non-lactose fermenting, Gram-negative rods that are H2S positive on TSI agar. Which pathogen is most likely responsible?

A) Shigella sonnei
B) Enterotoxigenic E. coli (ETEC)
C) Vibrio cholerae
D) Salmonella Enteritidis


4. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Detection of common antibiotic resistance genes not currently expressed.
B) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).
C) Presence of non-toxigenic C. difficile strains.
D) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.


5. A patient develops a febrile illness with a diffuse petechial rash after a camping trip. Serological testing reveals a four-fold rise in antibody titer to Rickettsia rickettsii. The vector responsible for transmitting this organism is a:

A) Mosquito
B) Louse
C) Tick
D) Flea


Solutions:

Question # 1
Answer: D
Question # 2
Answer: B
Question # 3
Answer: D
Question # 4
Answer: B
Question # 5
Answer: C

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